Some of the aromatic compounds and lower fatty acids are mechanically carried over in the surroundings by the (medicinal fumes) of yagya, which acts as a colloidal vehicle.
The combustion of fatty substances gives a number of hydrocarbons and other chemicals: from glycerol portion are obtained acetone bodies, pyruvic aldehyde, glycol, glyoxal etc, most of which are anti-bacterial.
More than hundred phytochemcicals found in the plant medicines used in above yagya have significant anti-microbial activity.
Over twenty other phytochemicals with strong anti-tubercular activity, namely are found as most likely constituents of the medicinal fumes/gases/vapors of yagya.
The temperature being sufficiently high and varying between wide limits, the substances with boiling points between 200-350 degree (iC) vaporize and diffuse out. This class includes various oils from sandalwood, agars, sesame, deodar, various terpens and aromatic compounds of high boiling points. Large number of terpens (such as borneol, gereniol, nerol, terpeneol, etc) are found in yagya-output. Many of these are also reported to have anti-mycobacterial activity.
The accepted models of different modes of action of terpenes against bacteria suggest that the terpenes could Interfere with the phospholipids bilayer of the cell membrane, Impair a variety of enzyme (HMG-reductase); Impair and Destroy or inactivate genetic material. Although chemically different, the second reaction would be similar in its effect, to Isoniazid activity via catalase-perosidase KatG.
Importance of Using Whole Herb Apart from synergy there are many sound reasons to explain the difference between the actions of isolating chemicals and that of whole herbs. For example, sometimes the presence of the whole plant material, which may contain antioxidants, may 'protect' the actives from decomposition; similarly, the essential oils released during yagya work as carriers for the active compounds to reach the lungs via inhalation.
The oxidation of the hydrocarbons in the cellulose-rich wood and the plant medicines used in yagya produces carbon dioxide and water in controlled amounts so that these could support co-administration of charcoal (and thus prevent oral absorption of the medicines inhaled during yagya) and also act as rejuvenating stimulants.
Mycolic acid has long been a popular anti-tuberculosis drugs target. Mycobacteirum Tuberculosis possesses a remarkable range of lipophilic molecules unrivalled in the prokaryotic world. New discoveries reported in literature open a new field to investigation of drug targeting where the primary targets are not crucial enzymes involved in biosynthesis but rather crucial enzymes involved in degradation. Strong possibility of presence of lots of liphophilic compounds in yagya-output and some of which in view of recent discoveries on related genes, can inhibit the growth of M. Tuberoculosis, further supports the therapeutic potential of yagya.